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The size itself is the complication in chromatography: a short peptide elutes early in reversed phase, in the region where solvent-front artifacts, residual synthesis reagents and truncated sequences also elute, so co-elution of impurities with the main peak is a realistic concern rather than a theoretical one

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Citation Andres E, Zulfiqar AA, Vogel T, Kaltenbach G (2018) State of art of oral vitamin B12 (cobalamin) treatment in Biermer's disease (pernicious anemia)